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Title: Improving the Quality of Stored Blood by Low-Level Laser Irradiation prior to Transfusion Procedures: An In Vitro Study
Author(s): Dan-Georgel SipoÅŸan   Petrică Ciotîrnae   Răzvan-Doru Raicu         

Abstract: The purpose of the current study was to clarify whether or not the method of low-laser irradiation of stored blood could be a prospect method in transfusion hematology, in order to improve the quality of stored blood. We irradiated blood from different donors, preserved in MacoPharma-type bags, using various irradiation sources in the spectral range of 630-990 nm. We looked for the dependence of the most important blood parameters characteristic for the preservation process, i.e., rheologic, plasmatic ions, osmotic resistance, viscosity, free hemoglobin, and erythrocyte functional morphology, as a function of laser parameters: wavelength, irradiating dose, mode of laser functioning, and frequency of modulation. The most important results consisted in the beneficial effects, such as decreasing the level of K+ plasmatic ions, mean corpuscular volume and free hemoglobin, as well as the increase of osmotic globular resistance, for the irradiated blood, using some particular protocols of irradiation. The most remarkable effects occurred in the case of effectively received doses between 0.1 and 1.0 J•cm–3 in a continuous functioning mode of irradiation, and of doses around one order lower in pulsed functioning mode. The main conclusion drawn from the results is that the most efficient irradiation aiming at rejuvenation of stored blood was that performed with infrared pulsed matrices, on large bags. The advantages of the proposed method – low cost, non-noxious or harmful, easy to implement, and closed system procedure – recommend it to be part of the current transfusion practice probably not before long.

Keywords: low-level laser irradiation, free hemoglobin, osmotic resistance, rejuvenation.

[1] W.J. WILLIAMS (Ed.) – Williams Hematology Companion Handbook, McGraw-Hill, New York, NY, 1996
[2] D. BRATOSIN, L. MITROFAN, C. PALII, J. ESTAQUIER, J. MONTREUIL – Novel Fluorescence Assay Using Calcein-AM for the Determination of Human Erythrocyte Viability and Aging, Cytometry Part A, Vol. 66A, No. 1, pp. 78-84, Jul. 2005
[3] I.G. DUTKEVICH, A.V. MARCHENKO – Photo-Hemotherapy, In E.P. Svedentzova (Ed.), “A Textbook of Transfusional Medicine”, pp. 549-568, State Institution Publishing House, Kirov, Russia, 1999 (in Russian)
[4] K.J. DILLON – Healing Photons: The Science and Art of Blood Irradiation Therapy, Scientia Press, Washington, D.C., 1998
[5] V.I. KARANDASHOV, E.B. PETUHOV, V.S. ZRODNIKOV – Phototherapy: A Guide for Physicians, Meditzina Publishing House, Moscow, Russia, 2001 (in Russian)
[6] A.V. MARCHENKO, I.G. DUTKEVICH, A.Kh. MA’LSAGOV, G.V. GOLOVIN – The Role of Daylight on the Blood in the Mechanism of the Therapeutic Effect of Blood Transfusion, Vestn Khir Im I I Grek, Vol. 144, No. 8, pp. 114-117, 1989 (in Russian)
[7] R.B. JOHNSON, P.A. NAPYCHANK, S. MURPHY, E.L. SNYDER – In Vitro Changes in Platelet Function and Metabolism Following Increasing Doses of Ultraviolet-B Irradiation, Transfusion, Vol. 33, No. 3, pp. 249-255, Mar. 1993
[8] K. YOKOYAMA, K. SUGIYAMA – Influence of Linearly Polarized Near-Infrared Irradiation on Deformability of Human Stored Erythrocytes, Journal of Clinical Laser Medicine & Surgery, Vol. 21, No. 1, pp. 19-22, Feb. 2003
[9] K.A. SAMOĬLOVA, S.A. SNOPOV, K.D. OBOLENSKAIA, R.A. ARTSISHEVSKAIA, A.V. VOLOGDINA, V.I. SHTIL’BANS – Trigger Mechanisms of the Therapeutic Effects of Autologous Transfusion of UV-Irradiated Blood: Membranotropic Effect on Erythrocytes and Thrombocytes, Vestn Khir Im I I Grek, Vol. 144, No. 12, pp. 51-60, 1989 (in Russian)
[10] I.G. DUTKEVICH, G.V. GOLOVIN, A.V. MARCHENKO, G.E. ZHUKHINA, K.A. SAMOĬLOVA – Effect of the Autotransfusion of UV-Irradiated Blood on the Homeostatic Indices of Surgical Patients, Vestn Khir Im I I Grek, Vol. 138, No. 1, pp. 59-65, 1987 (in Russian)
[11] K.A. SAMOĬLOVA, S.A. SNOPOV, N.K. BELISHEVA, L.M. KUKUĬ, I.E. GANELINA – Functional and Structural Changes in the Surface of Human Erythrocytes after Irradiation by Different Wave Lengths of UV Rays. Part III: The Immediate Effect of the Autotransfusion of UV-Irradiated Blood, Tsitologiia, Vol. 29, No. 7, pp. 810-817, 1987 (in Russian)
[12] F.K.H. KUTUSHEV, V.V. CHALENKO, V.L. VANEVSKIĬ et al. – Photomodification of Autologous Blood in Surgery, Khirurgiia, Vol. 11, pp. 104-108, 1990 (in Russian)
[13] A.E. GROMOV, S.A. SNOPOV, K.A. SAMOĬLOVA, L.M. KUKUĬ, I.E. GANELINA – Functional and Structural Changes in the Surface of Human Erythrocytes after UV Irradiation with Rays of Various Wavelengths. Part IV: Changes in the Physicochemical Properties of Autotransfused UV-Irradiated Blood, Tsitologiia, Vol. 30, No. 12, pp. 1442-1448, 1988 (in Russian)
[14] L.N. VLADIMIROVA – The Influence Mechanisms of Low-Intensity Laser Radiation on the Hemostasis System and Microcirculation, Ph.D. Thesis, Saint-Petersburg State University, Russia, 2000 (in Russian)
[15] L.V. GASPARYAN – Intravenous Laser Blood Irradiation Therapy, Healing Photons,
[16] T. KARU – The Science of Low-Power Laser Therapy, Gordon and Breach Science Publishers, Amsterdam, The Netherlands, 1998
[17] N.N. KIPSHIDZE, G.E. CHAPIDZE, I.M. KOROCHKIN, M.R. BOHUA, L.A. MARSAGISHVILI, G.M. KAPUSTINA – HeNe Laser Treatment of Heart Ischemic Disease, Amirani Publishing House, Tbilisi, Georgia, 1993 (in Russian)
[18] O.I. VORONINA, M. KAPLAN, V.A. STEPANOV – The Non-Resonant Mechanism of the Biostimulating Effect Concerning the Low-Intensity Laser Radiation, Physical Medicine, Vol. 2, No. 1-2, pp. 40-50, 1992 (in Russian)
[19] J. TUNÉR, L. HODE – Laser Therapy: Clinical Practice and Scientific Background, Prima Books, Grängesberg, Sweden, 2002
[20] V.A. BUILIN – Low-Intensity Laser Therapy Using Pulsed Laser Matrices: Informative Methodical Textbook, TOO Firm TEHNIKA, Moscow, Russia, 2000 (in Russian)
[21] T. KARU – Ten Lectures on Basic Science of Laser Phototherapy, Prima Books, Grängesberg, Sweden, 2007
[22] R.D. SAFAEV – Selected Methods of Laser Treatment: The Essential Diseases and Pathological States, Azor Publishing House, Moscow, Russia, 2001 (in Russian)
[23] Y. CUI, Z. GUO, Y. ZHAO, Y. ZHENG, Y. QIAO, J. CAI, S. LIU – Reactive Effect of Low Intensity He-Ne Laser upon Damaged Ultrastructure of Human Erythrocyte Membrane in Fenton System by Atomic Force Microscopy, Acta Biochimica et Biophysica Sinica, Vol. 39, No. 7, pp. 484-489, 2007
[24] R.X. do NASCIMENTO, F. CALLERA – Low-Level Laser Therapy at Different Energy Densities (0.1-2.0 J/cm2) and Its Effects on the Capacity of Human Long-Term Cryopreserved Peripheral Blood Progenitor Cells for the Growth of Colony-Forming Units, Photomedicine and Laser Surgery, Vol. 24, No. 5, pp. 601-604, Oct. 2006
[25] X.Q. MI, J.Y. CHEN, L.W. ZHOU – Effect of Low Power Laser Irradiation on Disconnecting the Membrane-Attached Hemoglobin from Erythrocyte Membrane, Journal of Photochemistry and Photobiology B: Biology, Vol. 83, No. 2, pp. 146-150, May 2006
[26] X.Q. MI, J.Y. CHEN, Z.J. LIANG, L.W. ZHOU – In Vitro Effects of Helium-Neon Laser Irradiation on Human Blood: Blood Viscosity and Deformability of Erythrocytes, Photomedicine and Laser Surgery, Vol. 22, No. 6, pp. 477-482, Dec. 2004
[27] M.A. VASILIEVICH – Intravascular Blood Photomodification in Surgery and Related Areas, Ph.D. Thesis, Saint-Petersburg State University, Russia, 1999 (in Russian)
[28] M.S.S. LEONART, A.J. NASCIMENTO, K. NONOYAMA, C.B. PELISSARI, A.E.M. STINGHEN, O.C. de O. BARRETTO – Correlation of Discocyte Frequency and ATP Concentration in Preserved Blood: A Morphological Indicator of Red Blood Cell Viability, Brazilian Journal of Medical Biological Research, Vol. 30, No. 6, pp. 745-747, Jun. 1997
[29] T. HOVAV, S. YEDGAR, N. MANNY, G. BARSHTEIN – Alteration of Red Cell Aggregability and Shape During Blood Storage, Transfusion, Vol. 39, No. 3, pp. 277-281, 1999
[30] T. VICSEK – Fractal Growth Phenomena, World Scientific, Singapore, Republic of Singapore, 1989
[31] Iu.L. SEVSCHENKO, S.A. MATVEEV, A.B. CECETKIN – Cardio-Surgical Transfusiology, Classic-Consulting, Moscow, Russia, 2000 (in Russian)
[32] N.A. ZHEVAGO, K.A. SAMOĬLOVA, K.D. OBOLENSKAIA, D.I. SOKOLOV – Changes in Cytokine Content in the Peripheral Blood of Volunteers after Their Exposure to Polychromatic Visible and Infrared Light, Tsitologiia, Vol. 47, No. 5, pp. 450-463, 2005 (in Russian)